Protein Purification
Cell Signaling Technology SimpleChIPR Human HoxA9 Prom
SimpleChIP(R) Human HoxA9 Promoter Primers 500 ul
Zymo Research Corporation Zymo-Spin™ ChIP Kit (10 preps) Mechanical Shearing Protocol '*'
This ChIP protocol involves covalent cross-linking of protein-DNA complexes with formaldehyde followed by cell lysis and chromatin shearing. A ChIP-grade antibody (user supplied) is used with Protein A magnetic beads to immunoprecipitate the protein-DNA complexes of interest. Following reverse crosslinking, RNase A and Proteinase K treatments, the DNA is eluted in a minimal volume of buffer using a unique micro-elution spin column, eliminating the need for messy precipitations. The protocol has been optimized for efficient crosslinking, shearing, and immunoprecipitation regardless of the mammalian cell type. Additionally, eluted ChIP DNA is ideal for end point PCR, quantitative PCR, ChIPSeq library preparation, and Next-Gen sequencing-based applications.
ABclonal Technology Acetyl-Histone H3-K27
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A H2B H3 and H4) The chromatin fiber is further compacted through the interaction of a linker histone H1 with the DNA between the nucleosomes to form higher order chromatin structures This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family Transcripts from this gene lack polyA tails instead they contain a palindromic termination element This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21 3
ABclonal Technology TriMethyl-Histone H3-K27
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A H2B H3 and H4) The chromatin fiber is further compacted through the interaction of a linker histone H1 with the DNA between the nucleosomes to form higher order chromatin structures This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family Transcripts from this gene lack polyA tails instead they contain a palindromic termination element This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21 3
Cell Signaling Technology SimpleChIPR Human c-Myc Intr
SimpleChIP(R) Human c-Myc Intron 1 Primers 500 ul
Cell Signaling Technology SimpleChIPR Mouse B-Myb Intr
SimpleChIP(R) Mouse B-Myb Intron 2 Primers 500 ul
Cell Signaling Technology SimpleChIP(R) Human MX1 Promoter Primers 500 ul
SimpleChIP(R) Human MX1 Promoter Primers 500 ul
ABclonal Technology TriMethyl-Histone H3-K27
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A H2B H3 and H4) The chromatin fiber is further compacted through the interaction of a linker histone H1 with the DNA between the nucleosomes to form higher order chromatin structures This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family Transcripts from this gene lack polyA tails instead they contain a palindromic termination element This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21 3
Cell Signaling Technology SimpleChIPR Mouse ATF-3 Intr
SimpleChIP(R) Mouse ATF-3 Intron 1 Primers 500 ul
Cell Signaling Technology SimpleChIP(R) Human SF3B3 Exon 1 Primers 500 ul
SimpleChIP(R) Human SF3B3 Exon 1 Primers 500 ul
Cell Signaling Technology SimpleChIP(R) Mouse TXNIP Promoter Primers 500 ul
SimpleChIP(R) Mouse TXNIP Promoter Primers 500 ul
Active Motif Siliconized Tubes, 1.7 ml
Because polystyrene transfers sonic wave energy much more efficiently than polypropylene, Active Motif highly recommends that its Polystyrene Sonication Tubes be used with its Q800R Sonicator. Use of polypropylene tubes in indirect sonication has been shown to reduce the extent to which the chromatin is sheared
Abcam Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA, 48 Tests
Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (ab117133) enables the user to enrich methylated DNA by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP).
The product is subject to the following: Abcam Restricted Use Statement
Cell Signaling Technology SimpleChIPR Mouse Sox2 Exon1
SimpleChIP(R) Mouse Sox2 Exon1 Primers 500 ul